Services

EnquBio's Comprehensive Drug Discovery Solutions & Services

At EnquBio, our expert team delivers high quality, efficient, and dependable R&D solutions that enable pharmaceutical and biotech partners to advance and expand their drug development pipelines.

Blood, PBMC & Primary Immune Cell Pharmacology

Human-relevant functional pharmacology using whole blood, PBMCs and primary immune-cell populations.

EnquBio develops functional ex vivo studies to characterize immune modulation, pathway responses, target-dependent activity and pharmacodynamic effects in primary immune systems. Studies can combine cellular, soluble-protein, protein-signaling and molecular readouts within a single design.

Blood, PBMC & Primary Immune Cell Pharmacology

Primary Immune Pharmacology in Biologically Relevant Systems

Study drug activity in whole blood, PBMCs and purified immune-cell populations using functional and mechanistic endpoints.

Human-Relevant Biology

Whole blood & PBMC assays

Evaluate compound activity in complex primary-cell systems that retain native immune-cell interactions.

Cell-Specific Pharmacology

Purified immune-cell populations

Interrogate T cells, B cells, monocytes, pDCs and other primary populations with targeted functional assays.

Cross-Species Support

Preclinical translation

Bridge human pharmacology with cynomolgus monkey, dog, rat and mouse studies where appropriate.

Representative Assay Areas

Assays are selected around pathway biology, cell type and the decision the study needs to support.

T-Cell Pharmacology

  • Activation and proliferation
  • Differentiation and exhaustion
  • Cytokine production
  • Functional modulation

B-Cell Pharmacology

  • B-cell activation
  • Memory B-cell responses
  • Plasmablast differentiation
  • Antibody production

Innate Immune Pharmacology

  • Monocyte activation
  • TLR pathway responses
  • pDC / type I IFN biology
  • Inflammatory cytokines

Whole-Blood Pharmacology

  • Stimulation / challenge assays
  • Cytokine response
  • Target engagement
  • Cellular pharmacodynamics

Typical Study Workflow

01

Sample Selection

Whole blood, PBMCs or purified immune cells.

02

Compound Exposure

Dose range, pretreatment or combination design.

03

Immune Challenge

Stimulate the relevant pathway or cell function.

04

Multi-Modal Readouts

Flow, MSD, Jess/Western and molecular assays.

05

Interpretation

Connect pharmacology with mechanism and biology.

Integrated Readouts

Flow CytometryPhenotype, activation, differentiation, signaling
MSD / ELISACytokines and soluble biomarkers
Jess / WesternProtein expression and pathway modulation
RT-qPCR / ddPCRGene expression and molecular PD
Functional EndpointsProliferation, differentiation, killing and response

Planning a primary immune-cell pharmacology study?

Share your target, cell population, compound mechanism and key biological question. Our scientists can help select the right primary-cell system and functional readouts.

Contact Our Scientists →
Whole blood, PBMC and purified immune-cell assays
Human and preclinical species support
Flow, MSD, Jess/Western and molecular readouts