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In Vitro Immunology Assays 

The intricate nature of the immune system poses challenges in developing therapies to modulate immune responses. Yet, this intricacy also unveils numerous therapeutic targets. Our team of expert immunologists address these challenges by offering in vitro immunology assays with primary human and rodent cells. These assays facilitate comprehensive immunological and pharmacological studies, providing deeper insights into your drug mechanisms and effects.

  1. T Cell Assays

    • T cell activation/proliferation assay

    • T cell differentiation assay (Th1, Th2, Th17 and Treg)​

  2. B Cell Assays

    • B cell activation/proliferation assay

    • B cell assay for antibody production

    • B cell antibody class switching assay

    • B cell antigen presentation assay

  3. Dendritic Cell Assays

    • DC differentiation assay

    • DC activation assay

    • Plasmacytoid DC isolation and activation assay

  4. Macrophage Assays

    • M1/M2-like macrophage differentiation

    • M1/M2-like macrophage repolarization

    • Migration/chemotaxis assay

  5. Immune Cell Co-Culture Assays

    • Mixed lymphocyte reaction (MLR) assay

    • Treg T cell suppression assay

    • M2-like macrophage T cell suppression assay

    • MDSC T cell suppression assay

  6. ​In vitro cytotoxicity assay

    • Primary NK cell induced cytotoxicity assay 

    • Antibody- dependent cellular phagocytosis assay (ADCP)

    • Complement- dependent cytotoxicity assay (CDC)

    • PBMC tumor cell co-culture assay

  7. Other Immune Cell Assays

    • Platelet activation assay

    • pSTAT1/2/3/4/5/6 activation assay (PBMC, whole blood)

Bioanalytical and Biomarker Platform

  1. Advanced multicolor flow cytometry analysis (human and mouse)

  2. MSD cytokine/chemokine analysis (multiplex)

  3. Western blot by Protein Simple Jess (fully automatic)

  4. Real-time qPCR (96- and 384- well format)

  5. In-house PBMC banking with immune cell phenotyping, such as T cells, B cells, NKs and etc.

  6. Immune cell isolation using beads or sorter, including T cells, B cells, NKs, monocytes, and DCs (human and mouse)

  7. In vitro evaluation for isolated cells (surface marker expression and co-culture of immune cells with tumor cells)

  8. Clinical sample analysis

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